Department of Forestry and Environmental Science
UNIVERSITY OF SRI JAYEWARDENPURA, SRI LANKA ../
FORESTRY AND ENVIRONMENT SYMPOSIUM
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|FORESTRY SYMPOSIUM 1996|
D M S H K Ranasinghe and G M Berlyn
Multiple shoot formation was induced from excised seedling nodal explants. On Murashige & Skoog medium supplemented with IBA (0.3 mg/litre) and BAP (1 mg/litre) about 2-5 shoots were obtained within 10-15 days from each explant, in a growth chamber at 25°C with a light source of 100 lux for 16 hours/day. When the shoots produced in vitro were subcultured to multiply on fresh medium of the same constituents, they yielded a crop of shoots within 3 weeks.
Shoot explants and tender leaf discs taken from plantlets grown in vitro and also from potted seedlings 3-4 months old from the growth chamber were also tried for adventitious shoot production without much success. Callus formation was also tried with these shoot explants.
Isolated shoots were rooted by culturing on the following media for 10 days: (a) half-strength MS medium devoid of growth regulators, or (b) half-strength MS medium supplemented with indolebutryic acid at (i) 0.3 mg/litre, (ii) 1 mg/litre, (iii) 2 mg/litre, or (iv) 3 mg/litre. They were then transferred to hormone-free half-strength MS medium. Roots were initiated in about 80% of the shoots in treatments b (i) and b (ii) while other treatments produced no rooting. However, when transferred to hormone-free medium, shoots that had been growing in treatments b (iii) and b (iv) also rooted within 7 days. The plantlets were hardened and transferred to pots, which were placed first in the growth chamber at 25°C with 100 lux for 16 hours/day and subsequently transferred to the greenhouse.
Department of Forestry and Environmental Science,