IN VITRO MULTIPLICATION AND ROOTING OF Artocarpus heterophyllus
K.A.H.K. Kasthuri Arachchi and W.T.P.S.K. Senarath
Department of Botany,
University of Sri Jayewardenepura
Artocarpus heterophyllus is an out breeding species. Therefore germination
of seeds may give seedlings with vast genetic diversity. Clonal propagation
of selected genotypes is very useful in maintaining selected characters.
Apical meristem from 20-30 years old jak plants were cultured in modified
Campbell and Durzan (CD) medium supplemented with 0.5mg/l Indole Butyric
Acid (IBA) in order to elongate shoots in vitro. After six weeks of incubation
in 25±10C (16 hr day), 60% of the explants produced elongated shoots.
They were cultured on Murashige & Skoog (MS) medium containing 1.5mg/l
IBA and 0.5mg/l kinetin for shoot multiplication and 4-5 shoots per explant
were obtained after five weeks of incubation. Non-multiplied shoots were
transferred into ½ MS medium containing 1.5mg/l IBA and 1.0mg/l Naphthalene
Acetic Acid (NAA) after five weeks. If shoots were kept in the same medium
(½ MS medium containing 1.5mg/l IBA + 1.0mg/l NAA) for more than two weeks
they will produce callus instead of roots at the base. Therefore after
two weeks they were transferred into CD medium containing 0.5mg/l IBA
for rooting. Root initials were observed after 10 days of incubation.
Department of Forestry and Environmental Science,
of Sri Jayewardenepura,Sri Lanka. 1995-2000. All rights reserved.