OF LUNUMIDELLA Melia dubia USING INVIVO AND INVITRO CULTURE METHODS
Visaka Seedevi Mapa, S E Peries and B C N Peiris
Faculty of Agriculture, University of Peradeniya
Lunumidella (Melia dubia) is a fast growing short rotational tree species
which can be harvested in 15-20 yrs. The timber is light and mainly used
for ceilling and panelling. However due to the presence of a hard seed
coat and limited period of viability planting of Lunumidella has become
Therefore investigations were carried out with the objective of finding
suitable propagation techniques using invivo and invitro culture methods.Shoot
tip culture method was carried out using axillary buds and nodel segments
taken from plant materials brought from the Dodangolla University farm
and kept under the running tap water for 2 hours. Then surface sterilization
was done using 10%. Naocl containing 2 drops of teepol/100ml solution
for 10 mts. in sterilized conditions. They were then washed with distilled
water 3 times for 2 min. each and transferred into Mureshige and skoog
medium (MS) containing different concentrations of cytokinins.
The best medium to grow Lunumedella invitro was 1/2 strength Ms Agar medium
with the supplement of 1.0 mg/l BAP.
In vivo methods
Different pretreatment methods were adopted to overcome the poor germination
due to the hard seed coat by using acid treatments, base treatments, heat
treatments and mechanical scarification. Seeds were treated with 98% H2
SO4 for different time periods; 1 mt, 5 mts., 10mts, 15 mts, 30mts) and
then soaked in water for 24 hours and planted them in poly bags in the
Likewise the same procedure was carried out with base Ca(OH)2 and KOH
The best pretreatment method for Lunumidella was acid pretreatment method.
In this case the germination percentage was 40%.
Department of Forestry and Environmental Science,
of Sri Jayewardenepura,Sri Lanka. 1999. All rights reserved.